Liam Cooper, Greg Taylor and Jacob Brown have been recalled to the Scotland squad for the Euro 2024 qualifier in Spain.
Hearts striker Lawrence Shankland drops out along with, as expected, Newcastle midfielder Elliot Anderson.
Whitley Bay-born Anderson last week indicated he wanted more time to consider his international future, having left the last Scotland camp after two days following his first call-up to the full squad.
Leeds defender Cooper returns from injury while Celtic left-back Taylor replaces Kieran Tierney, who suffered a hamstring injury while playing for Real Sociedad on Saturday.
Luton striker Brown is yet to score in the Premier League since his move from Stoke, while Shankland has gone seven matches without scoring after hitting five goals in his first six games of the season for Hearts.
Scotland have won their opening five games and are six points ahead of second-placed Spain ahead of their trip to Seville on October 12.
Steve Clarke’s side then face France in a friendly in Lille five days later.